Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2542349..2542846 Replicon chromosome
Accession NZ_CP072802
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J8617_RS12415 Protein ID WP_001173343.1
Coordinates 2542349..2542522 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag J8617_RS12420 Protein ID WP_000398619.1
Coordinates 2542550..2542846 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS12405 (2539019) 2539019..2540476 + 1458 WP_000856801.1 dipeptide/tripeptide permease DtpC -
J8617_RS12410 (2540713) 2540713..2542230 + 1518 WP_001295074.1 lysine--tRNA ligase -
J8617_RS12415 (2542349) 2542349..2542522 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J8617_RS12420 (2542550) 2542550..2542846 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J8617_RS12425 (2543073) 2543073..2543345 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
J8617_RS12430 (2543357) 2543357..2543587 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
J8617_RS12435 (2543768) 2543768..2545399 + 1632 WP_001216477.1 sensor histidine kinase -
J8617_RS12440 (2545396) 2545396..2546115 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198907 WP_001173343.1 NZ_CP072802:c2542522-2542349 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198907 NZ_CP097056:c2569585-2569484 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT198907 WP_000398619.1 NZ_CP072802:c2542846-2542550 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198907 NZ_CP097056:2569365-2569539 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References