Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2355233..2355809 Replicon chromosome
Accession NZ_CP072802
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag J8617_RS11490 Protein ID WP_024184631.1
Coordinates 2355522..2355809 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag J8617_RS11485 Protein ID WP_072841999.1
Coordinates 2355233..2355535 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS11465 (2350924) 2350924..2351436 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
J8617_RS11470 (2351829) 2351829..2353979 + 2151 WP_001309197.1 pyruvate/proton symporter BtsT -
J8617_RS11475 (2354029) 2354029..2354232 + 204 WP_000467859.1 YbdD/YjiX family protein -
J8617_RS11480 (2354243) 2354243..2355199 + 957 WP_001295525.1 GTPase -
J8617_RS11485 (2355233) 2355233..2355535 - 303 WP_072841999.1 BrnA antitoxin family protein Antitoxin
J8617_RS11490 (2355522) 2355522..2355809 - 288 WP_024184631.1 BrnT family toxin Toxin
J8617_RS11495 (2356006) 2356006..2357172 + 1167 WP_000800826.1 restriction endonuclease -
J8617_RS11500 (2357236) 2357236..2358855 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
J8617_RS11505 (2358845) 2358845..2359606 + 762 Protein_2280 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11184.68 Da        Isoelectric Point: 7.4697

>T198905 WP_024184631.1 NZ_CP072802:c2355809-2355522 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
VRKADRKERSRYEHG

Download         Length: 288 bp

>T198905 NZ_CP097056:c341123-341028 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11546.05 Da        Isoelectric Point: 9.4646

>AT198905 WP_072841999.1 NZ_CP072802:c2355535-2355233 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT198905 NZ_CP097056:340929-340993 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References