Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1802360..1802978 Replicon chromosome
Accession NZ_CP072802
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J8617_RS08860 Protein ID WP_001291435.1
Coordinates 1802760..1802978 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J8617_RS08855 Protein ID WP_000344800.1
Coordinates 1802360..1802734 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS08845 (1797449) 1797449..1798642 + 1194 WP_001351640.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J8617_RS08850 (1798665) 1798665..1801814 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J8617_RS08855 (1802360) 1802360..1802734 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J8617_RS08860 (1802760) 1802760..1802978 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J8617_RS08865 (1803150) 1803150..1803701 + 552 WP_000102564.1 maltose O-acetyltransferase -
J8617_RS08870 (1803817) 1803817..1804287 + 471 WP_001351639.1 YlaC family protein -
J8617_RS08875 (1804451) 1804451..1806001 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J8617_RS08880 (1806043) 1806043..1806396 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J8617_RS08890 (1806775) 1806775..1807086 + 312 WP_000409911.1 MGMT family protein -
J8617_RS08895 (1807117) 1807117..1807689 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T198902 WP_001291435.1 NZ_CP072802:1802760-1802978 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T198902 NZ_CP097049:c11880-11611 [Enterococcus faecalis]
ATGTCGTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT198902 WP_000344800.1 NZ_CP072802:1802360-1802734 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT198902 NZ_CP097049:c12107-11880 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGTGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References