Detailed information of TA system TA198882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-higA/LOTUS_5_Limkain_b1-MqsA
• Location: 3186561..3187357
• Replicon: chromosome
• Accession: NZ_CP072800
• Organism: Candidatus Thiothrix anitrata isolate A52

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J8380_RS15925
• Protein ID: WP_210226533.1
• Coordinates: 3186561..3186956 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J8380_RS15930
• Protein ID: WP_210226534.1
• Coordinates: 3187043..3187357 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8380_RS15870 (J8380_15870)	3182294..3182539	-	246	WP_210226522.1	helix-turn-helix transcriptional regulator	-
J8380_RS15875 (J8380_15875)	3182622..3182798	+	177	WP_210226523.1	hypothetical protein	-
J8380_RS15880 (J8380_15880)	3182813..3183046	+	234	WP_210226524.1	hypothetical protein	-
J8380_RS15885 (J8380_15885)	3183225..3183461	-	237	WP_210226525.1	UPF0175 family protein	-
J8380_RS15890 (J8380_15890)	3183475..3183717	-	243	WP_210226526.1	hypothetical protein	-
J8380_RS18430	3183727..3183855	-	129	WP_266097288.1	hypothetical protein	-
J8380_RS15895 (J8380_15895)	3184009..3184287	+	279	WP_210226527.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
J8380_RS15900 (J8380_15900)	3184284..3184721	+	438	WP_210226528.1	type II toxin-antitoxin system VapC family toxin	-
J8380_RS15905 (J8380_15905)	3184718..3184960	-	243	WP_210226529.1	UPF0175 family protein	-
J8380_RS15910 (J8380_15910)	3185026..3185262	-	237	WP_210226530.1	hypothetical protein	-
J8380_RS15915 (J8380_15915)	3185274..3186131	-	858	WP_210226531.1	Rpn family recombination-promoting nuclease/putative transposase	-
J8380_RS15920 (J8380_15920)	3186322..3186564	+	243	WP_210226532.1	DUF2281 domain-containing protein	-
J8380_RS15925 (J8380_15925)	3186561..3186956	+	396	WP_210226533.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8380_RS15930 (J8380_15930)	3187043..3187357	+	315	WP_210226534.1	hypothetical protein	Antitoxin
J8380_RS15935 (J8380_15935)	3187397..3187576	-	180	WP_210226535.1	hypothetical protein	-
J8380_RS15940 (J8380_15940)	3187652..3187954	-	303	WP_228292265.1	PIN domain-containing protein	-
J8380_RS15945 (J8380_15945)	3188076..3189710	-	1635	Protein_3205	chaperonin GroEL	-
J8380_RS15950 (J8380_15950)	3189730..3190020	-	291	WP_093069816.1	co-chaperone GroES	-
J8380_RS15955 (J8380_15955)	3190119..3190589	-	471	WP_210226537.1	FxsA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14597.77 Da
• Isoelectric Point: 6.3277

>T198882 WP_210226533.1 NZ_CP072800:3186561-3186956 [Candidatus Thiothrix anitrata]
MILVDTDVLIWVTRKNPHASAFLDSFDDLAISDVTYMELIQGAKNKREAQAIDKTLKNMAVSRLPINDSISERAVALVRR
YFHSHSMQLADALIAATALEYGLALATGNAKHFEVVEDLQLHKYTPQPTAM

Nucleotide

• Length: 396 bp

>T198882 NZ_CP097046:c308469-308374 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11933.70 Da
• Isoelectric Point: 10.4451

>AT198882 WP_210226534.1 NZ_CP072800:3187043-3187357 [Candidatus Thiothrix anitrata]
MSKRNLFEELSTALVEAKQHSEGKLTLRTHVVQRTGVPEIKPQEILTIREMFNMSRGVFARHLHTSARTLESWEQGRTTP
NGQAVTLLRLVQRHPETLSYIAEL

Nucleotide

• Length: 315 bp

>AT198882 NZ_CP097046:308275-308339 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT