Detailed information of TA system TA198881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2329562..2330586
• Replicon: chromosome
• Accession: NZ_CP072800
• Organism: Candidatus Thiothrix anitrata isolate A52

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: J8380_RS12000
• Protein ID: WP_210225863.1
• Coordinates: 2329562..2330140 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: J8380_RS12005
• Protein ID: WP_210225864.1
• Coordinates: 2330140..2330586 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8380_RS11960 (J8380_11960)	2325060..2325977	-	918	WP_228292458.1	TIGR02594 family protein	-
J8380_RS11965 (J8380_11965)	2325977..2326309	-	333	WP_210225856.1	hypothetical protein	-
J8380_RS11970 (J8380_11970)	2326309..2326509	-	201	WP_210225857.1	hypothetical protein	-
J8380_RS11975 (J8380_11975)	2326536..2327063	-	528	WP_210225858.1	hypothetical protein	-
J8380_RS11980 (J8380_11980)	2327060..2327314	-	255	WP_210225859.1	hypothetical protein	-
J8380_RS11985 (J8380_11985)	2327471..2328640	-	1170	WP_210225860.1	hypothetical protein	-
J8380_RS11990 (J8380_11990)	2328893..2329132	-	240	WP_210225861.1	hypothetical protein	-
J8380_RS11995 (J8380_11995)	2329202..2329537	+	336	WP_210225862.1	helix-turn-helix transcriptional regulator	-
J8380_RS12000 (J8380_12000)	2329562..2330140	-	579	WP_210225863.1	PIN domain-containing protein	Toxin
J8380_RS12005 (J8380_12005)	2330140..2330586	-	447	WP_210225864.1	helix-turn-helix domain-containing protein	Antitoxin
J8380_RS12010 (J8380_12010)	2330802..2331014	+	213	WP_210225865.1	hypothetical protein	-
J8380_RS12015 (J8380_12015)	2331011..2331433	+	423	WP_210225866.1	hypothetical protein	-
J8380_RS12020 (J8380_12020)	2331436..2331729	+	294	WP_210220247.1	hypothetical protein	-
J8380_RS12025 (J8380_12025)	2331861..2333027	+	1167	WP_210225867.1	hypothetical protein	-
J8380_RS12035 (J8380_12035)	2333378..2335258	-	1881	WP_210225868.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-116)

Antitoxin

• Helix-turn-helix domain, group 17 (78-126)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21976.52 Da
• Isoelectric Point: 8.8504

>T198881 WP_210225863.1 NZ_CP072800:c2330140-2329562 [Candidatus Thiothrix anitrata]
MLSGQNFTVLYDACVLYPAPLRDFLVRLAWTGVFRAKWTQQIHEEWIRNTLQNNTKLTRERLEMTAGKMNAAIMDSLVSG
YEPLIAGLELPDVNDRHVLAAAIKGQAEVIVTFNLKDFPASYLKPFGILAQHPDEFISHLTNLYPEMILLTARQQRASLK
NPPRTVDEFLDTLLRQQLPRTAAFLKQRKELI

Nucleotide

• Length: 579 bp

>T198881 NZ_CP097043:c31053-30712 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16625.30 Da
• Isoelectric Point: 5.2767

>AT198881 WP_210225864.1 NZ_CP072800:c2330586-2330140 [Candidatus Thiothrix anitrata]
MPALVSYQVPTQEDTELAVESSRLLAACIGKGESACLRLHNGNELLQVPVKAIRLLVDILDAMARGDAVSLIPIHKELTT
QEAANILNVSRPYLVKLMEAGEIPFHKNGVRRRVFFKDLMEYKQKRDQANMLLLDELTAEAQEFEMGY

Nucleotide

• Length: 447 bp

>AT198881 NZ_CP097043:c31283-31053 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA