Detailed information of TA system TA198877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1733953..1734623
• Replicon: chromosome
• Accession: NZ_CP072800
• Organism: Candidatus Thiothrix anitrata isolate A52

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J8380_RS09085
• Protein ID: WP_210230336.1
• Coordinates: 1734219..1734623 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J8380_RS09080
• Protein ID: WP_210230334.1
• Coordinates: 1733953..1734216 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8380_RS09055 (J8380_09055)	1731268..1731555	+	288	WP_210230316.1	DUF6516 family protein	-
J8380_RS09060 (J8380_09060)	1731537..1731902	+	366	WP_210230318.1	hypothetical protein	-
J8380_RS09065 (J8380_09065)	1731907..1732326	-	420	WP_210230328.1	PIN domain-containing protein	-
J8380_RS09070 (J8380_09070)	1732316..1732597	-	282	WP_210230330.1	hypothetical protein	-
J8380_RS09075 (J8380_09075)	1732809..1733813	-	1005	WP_210230332.1	SH3 domain-containing protein	-
J8380_RS09080 (J8380_09080)	1733953..1734216	+	264	WP_210230334.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J8380_RS09085 (J8380_09085)	1734219..1734623	+	405	WP_210230336.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8380_RS09090 (J8380_09090)	1734735..1735919	+	1185	WP_210230623.1	tryptophan synthase subunit beta	-
J8380_RS09095 (J8380_09095)	1735916..1736725	+	810	WP_210230338.1	tryptophan synthase subunit alpha	-
J8380_RS09100 (J8380_09100)	1736739..1737635	+	897	WP_210230340.1	acetyl-CoA carboxylase, carboxyltransferase subunit beta	-
J8380_RS09105 (J8380_09105)	1737632..1738855	+	1224	WP_210230342.1	bifunctional tetrahydrofolate synthase/dihydrofolate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	cap8E / bplB / bplA / ugd	1696243..1903503	207260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-126)

Antitoxin

• SpoVT-AbrB domain (19-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14900.08 Da
• Isoelectric Point: 5.5504

>T198877 WP_210230336.1 NZ_CP072800:1734219-1734623 [Candidatus Thiothrix anitrata]
MTNYRYLLDTNIISNLFREPQGNVYQHLQRVGEGAVCTSIIVAAELRFGAKKRNSPALTAWVEQILSMIDVLPFDAPADH
AYAEIRAYLEAAGQIIGPNDLLIAAHAWHLGLVAVTNNTDEFKRVPGLIVENWL

Nucleotide

• Length: 405 bp

>T198877 NZ_CP097042:c2583823-2583719 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTTAAATT
GCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10089.65 Da
• Isoelectric Point: 5.1051

>AT198877 WP_210230334.1 NZ_CP072800:1733953-1734216 [Candidatus Thiothrix anitrata]
MYMPNQEVNMLAERHVRLFRNGRNQAIRIPREFELAGNEAIIRREGNRLIIEALQRPSLLAFLATCKPSELDFPDIDNLP
PLDDVVF

Nucleotide

• Length: 264 bp

>AT198877 NZ_CP097042:2583599-2583786 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTAACAATCAGC
GCAATCAAAGCAATGGTAAACATACCAA