Detailed information of TA system TA198871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6045..6607
• Replicon: plasmid pTunz4
• Accession: NZ_CP072799
• Organism: Thiothrix unzii strain A1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: J9260_RS18500
• Protein ID: WP_210220943.1
• Coordinates: 6045..6347 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J9260_RS18505
• Protein ID: WP_202719288.1
• Coordinates: 6335..6607 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9260_RS18465 (J9260_18460)	1726..2868	+	1143	WP_246499851.1	replication initiation protein	-
J9260_RS18470 (J9260_18465)	2965..3279	+	315	WP_210220937.1	hypothetical protein	-
J9260_RS18475 (J9260_18470)	3653..3856	+	204	WP_210220938.1	DUF2158 domain-containing protein	-
J9260_RS18480 (J9260_18475)	3856..4122	+	267	WP_210220939.1	hypothetical protein	-
J9260_RS18485 (J9260_18480)	4198..4848	-	651	WP_210220940.1	DUF3800 domain-containing protein	-
J9260_RS18490 (J9260_18485)	5020..5262	-	243	WP_210220941.1	ribbon-helix-helix domain-containing protein	-
J9260_RS18495 (J9260_18490)	5313..5954	-	642	WP_210220942.1	AAA family ATPase	-
J9260_RS18500 (J9260_18495)	6045..6347	-	303	WP_210220943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J9260_RS18505 (J9260_18500)	6335..6607	-	273	WP_202719288.1	CopG family ribbon-helix-helix protein	Antitoxin
J9260_RS18510 (J9260_18505)	6733..7038	-	306	WP_210220944.1	hypothetical protein	-
J9260_RS18515 (J9260_18510)	7433..7795	+	363	WP_210220945.1	DUF2513 domain-containing protein	-
J9260_RS18520 (J9260_18515)	7922..8881	+	960	WP_210220946.1	HEPN domain-containing protein	-
J9260_RS18525 (J9260_18520)	8979..9650	+	672	WP_210220947.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18228	18228
	-	inside	Integron	-	-	3..18227	18224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-93)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11342.14 Da
• Isoelectric Point: 8.5314

>T198871 WP_210220943.1 NZ_CP072799:c6347-6045 [Thiothrix unzii]
MPSLIFSHTALLDLERLRAFLRAKNLPAARRAVVKIMQGLQALEQHPELGHTVEDRPEDYRELVIPFGKDGYIAAYRYAG
DSLVVLGVRHQRELQPPTLP

Nucleotide

• Length: 303 bp

>T198871 NZ_CP097038:2718213-2718356 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10848.12 Da
• Isoelectric Point: 5.5623

>AT198871 WP_202719288.1 NZ_CP072799:c6607-6335 [Thiothrix unzii]
MPTSIKLDMDTKTRLQRLATSRQRSTHWLMQEAIRQYLEREEQLAQFRDEMQTAWDDYQDTGLHVTGKEVFAWMETWFTD
SEAQTPKCHH

Nucleotide

• Length: 273 bp

>AT198871 NZ_CP097038:c2718550-2718288 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGACAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA