Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2216468..2217045 Replicon chromosome
Accession NZ_CP072793
Organism Thiothrix unzii strain A1

Toxin (Protein)


Gene name chpK Uniprot ID -
Locus tag J9260_RS11000 Protein ID WP_210217816.1
Coordinates 2216704..2217045 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID -
Locus tag J9260_RS10995 Protein ID WP_210217815.1
Coordinates 2216468..2216704 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9260_RS10965 (J9260_10960) 2212513..2212881 + 369 WP_210217812.1 helix-turn-helix domain-containing protein -
J9260_RS10970 (J9260_10965) 2213021..2213476 + 456 WP_210217479.1 transposase -
J9260_RS10975 (J9260_10970) 2213557..2214579 + 1023 WP_210217813.1 DUF2130 domain-containing protein -
J9260_RS10980 (J9260_10975) 2214631..2215851 + 1221 WP_210217517.1 IS256 family transposase -
J9260_RS10985 (J9260_10980) 2215869..2216093 - 225 WP_246499399.1 PIN domain-containing protein -
J9260_RS10990 (J9260_10985) 2216097..2216354 - 258 WP_210217814.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J9260_RS10995 (J9260_10990) 2216468..2216704 + 237 WP_210217815.1 hypothetical protein Antitoxin
J9260_RS11000 (J9260_10995) 2216704..2217045 + 342 WP_210217816.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J9260_RS11005 (J9260_11000) 2217122..2218183 - 1062 WP_210217817.1 class III poly(R)-hydroxyalkanoic acid synthase subunit PhaC -
J9260_RS11010 (J9260_11005) 2218213..2219370 - 1158 WP_210217818.1 class III poly(R)-hydroxyalkanoic acid synthase subunit PhaE -
J9260_RS11015 (J9260_11010) 2219476..2220660 + 1185 WP_210217819.1 acetyl-CoA C-acetyltransferase -
J9260_RS11020 (J9260_11015) 2220797..2221525 + 729 WP_210217820.1 acetoacetyl-CoA reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12543.62 Da        Isoelectric Point: 10.0937

>T198869 WP_210217816.1 NZ_CP072793:2216704-2217045 [Thiothrix unzii]
MKRGEIWWASLEEPRGSEPGYKRPVVIISSDAFNHSRIQTVIVAIVTSNLRLATAPGNISLAKRDTELLKESVINVSQIL
TIDKSFLIEKIGRLPDKKISALDEGLKLSLSIF

Download         Length: 342 bp

>T198869 NZ_CP097038:c2712112-2711771 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAGTCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8942.19 Da        Isoelectric Point: 4.5338

>AT198869 WP_210217815.1 NZ_CP072793:2216468-2216704 [Thiothrix unzii]
MKTAISIPDPIFQAAELFANHFGISRSELYAKAIEEYIASRRFMNVTAALNKIYGDEPSVLDDELYSMQIKSIPREEW

Download         Length: 237 bp

>AT198869 NZ_CP097038:c2712342-2712112 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References