Detailed information of TA system TA198861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1232571..1233488
• Replicon: chromosome
• Accession: NZ_CP072791
• Organism: Bacillus velezensis strain GS-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: J8615_RS06425
• Protein ID: WP_025851786.1
• Coordinates: 1232742..1233488 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: J8615_RS06420
• Protein ID: WP_003154807.1
• Coordinates: 1232571..1232741 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8615_RS06385 (1227802)	1227802..1229424	+	1623	WP_014304855.1	pyocin knob domain-containing protein	-
J8615_RS06390 (1229437)	1229437..1229745	+	309	WP_224979391.1	hypothetical protein	-
J8615_RS06395 (1229814)	1229814..1230011	+	198	WP_003154819.1	XkdX family protein	-
J8615_RS06400 (1230068)	1230068..1230829	+	762	WP_043867020.1	hypothetical protein	-
J8615_RS06405 (1230881)	1230881..1231144	+	264	WP_003154815.1	hemolysin XhlA family protein	-
J8615_RS06410 (1231158)	1231158..1231421	+	264	WP_003154813.1	phage holin	-
J8615_RS06415 (1231435)	1231435..1232313	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
J8615_RS20170 (1232349)	1232349..1232474	-	126	WP_003154809.1	hypothetical protein	-
J8615_RS06420 (1232571)	1232571..1232741	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
J8615_RS06425 (1232742)	1232742..1233488	-	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J8615_RS06430 (1233592)	1233592..1234590	-	999	WP_003154805.1	inorganic phosphate transporter	-
J8615_RS06435 (1234603)	1234603..1235220	-	618	WP_003154804.1	DUF47 domain-containing protein	-
J8615_RS06440 (1235506)	1235506..1236822	-	1317	WP_003154801.1	amino acid permease	-
J8615_RS06445 (1237145)	1237145..1238095	+	951	WP_052585757.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T198861 WP_025851786.1 NZ_CP072791:c1233488-1232742 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T198861 NZ_CP097034:c2675348-2675007 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT198861 WP_003154807.1 NZ_CP072791:c1232741-1232571 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT198861 NZ_CP097034:c2675578-2675348 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14