Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 475685..476322 Replicon chromosome
Accession NZ_CP072791
Organism Bacillus velezensis strain GS-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag J8615_RS02440 Protein ID WP_003156187.1
Coordinates 475972..476322 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag J8615_RS02435 Protein ID WP_003156188.1
Coordinates 475685..475966 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8615_RS02415 (472050) 472050..472649 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
J8615_RS02420 (472742) 472742..473107 + 366 WP_014304402.1 holo-ACP synthase -
J8615_RS02425 (473272) 473272..474279 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
J8615_RS02430 (474396) 474396..475565 + 1170 WP_025853726.1 alanine racemase -
J8615_RS02435 (475685) 475685..475966 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
J8615_RS02440 (475972) 475972..476322 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J8615_RS02445 (476440) 476440..477261 + 822 WP_014304404.1 STAS domain-containing protein -
J8615_RS02450 (477266) 477266..477631 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
J8615_RS02455 (477634) 477634..478035 + 402 WP_003156178.1 anti-sigma regulatory factor -
J8615_RS02460 (478047) 478047..479054 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
J8615_RS02465 (479118) 479118..479447 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
J8615_RS02470 (479444) 479444..479926 + 483 WP_003156173.1 anti-sigma B factor RsbW -
J8615_RS02475 (479892) 479892..480680 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
J8615_RS02480 (480680) 480680..481282 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T198860 WP_003156187.1 NZ_CP072791:475972-476322 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T198860 NZ_CP097034:c2516022-2515921 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT198860 WP_003156188.1 NZ_CP072791:475685-475966 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT198860 NZ_CP097034:2515798-2515976 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCG
AAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References