Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3698221..3698895 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag J8397_RS17550 Protein ID WP_003417282.1
Coordinates 3698221..3698649 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag J8397_RS17555 Protein ID WP_003417286.1
Coordinates 3698653..3698895 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS17525 (J8397_17330) 3694043..3694444 - 402 WP_003417264.1 cytidine deaminase -
J8397_RS17530 (J8397_17335) 3694681..3695019 + 339 WP_003417267.1 succinate dehydrogenase, cytochrome b556 subunit -
J8397_RS17535 (J8397_17340) 3695016..3695450 + 435 WP_003913036.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
J8397_RS17540 (J8397_17345) 3695579..3697351 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
J8397_RS17545 (J8397_17350) 3697351..3698142 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
J8397_RS17550 (J8397_17355) 3698221..3698649 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
J8397_RS17555 (J8397_17360) 3698653..3698895 - 243 WP_003417286.1 CopG family transcriptional regulator Antitoxin
J8397_RS17560 (J8397_17365) 3699017..3699631 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
J8397_RS17565 (J8397_17370) 3699628..3700293 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
J8397_RS17570 (J8397_17375) 3700294..3700827 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
J8397_RS17575 (J8397_17380) 3700824..3701198 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
J8397_RS17580 (J8397_17385) 3701295..3702431 - 1137 WP_003417297.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T198856 WP_003417282.1 NZ_CP072790:c3698649-3698221 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T198856 NZ_CP097033:46133-46402 [Enterococcus faecalis]
ATGTCGTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT198856 WP_003417286.1 NZ_CP072790:c3698895-3698653 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT198856 NZ_CP097033:45906-46133 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGGGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References