Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3129478..3130082 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag J8397_RS14960 Protein ID WP_003414492.1
Coordinates 3129478..3129870 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag J8397_RS14965 Protein ID WP_003414495.1
Coordinates 3129867..3130082 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS14930 (J8397_14740) 3124628..3125416 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
J8397_RS14935 (J8397_14745) 3125750..3126295 - 546 WP_003913758.1 DUF1802 family protein -
J8397_RS14940 (J8397_14750) 3126567..3127451 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
J8397_RS14945 (J8397_14755) 3127454..3128341 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
J8397_RS14950 (J8397_14760) 3128646..3129191 - 546 WP_009939112.1 DUF1802 family protein -
J8397_RS14955 (J8397_14765) 3129188..3129457 - 270 WP_003414489.1 DUF2277 family protein -
J8397_RS14960 (J8397_14770) 3129478..3129870 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
J8397_RS14965 (J8397_14775) 3129867..3130082 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J8397_RS14970 (J8397_14780) 3130129..3130878 + 750 WP_003414497.1 enoyl-CoA hydratase -
J8397_RS14975 (J8397_14785) 3130957..3132039 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
J8397_RS14980 (J8397_14790) 3132032..3133342 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
J8397_RS14985 (J8397_14795) 3133345..3134172 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
J8397_RS14990 (J8397_14800) 3134169..3135080 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T198852 WP_003414492.1 NZ_CP072790:c3129870-3129478 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T198852 NZ_CP097031:2660733-2660876 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT198852 WP_003414495.1 NZ_CP072790:c3130082-3129867 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT198852 NZ_CP097031:c2660622-2660480 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTT
TTAGTTACAAAAAATAACCGTACTCAGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References