Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3104311..3104881 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag J8397_RS14820 Protein ID WP_003414166.1
Coordinates 3104311..3104667 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag J8397_RS14825 Protein ID WP_003901465.1
Coordinates 3104651..3104881 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS14800 (J8397_14610) 3099763..3101451 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
J8397_RS14805 (J8397_14615) 3101455..3101781 - 327 WP_003414157.1 hypothetical protein -
J8397_RS14810 (J8397_14620) 3101954..3102541 + 588 WP_003914429.1 DUF3558 family protein -
J8397_RS14815 (J8397_14625) 3102560..3104209 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
J8397_RS14820 (J8397_14630) 3104311..3104667 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
J8397_RS14825 (J8397_14635) 3104651..3104881 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
J8397_RS14830 (J8397_14640) 3104924..3105967 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
J8397_RS14835 3105966..3106433 + 468 WP_003414177.1 DUF1778 domain-containing protein -
J8397_RS14840 (J8397_14650) 3106462..3106716 - 255 WP_003917684.1 hypothetical protein -
J8397_RS14845 (J8397_14655) 3106864..3107268 + 405 WP_009938577.1 hypothetical protein -
J8397_RS14850 (J8397_14660) 3107265..3107456 + 192 WP_003414184.1 hypothetical protein -
J8397_RS14855 3107673..3107933 + 261 Protein_2932 transposase -
J8397_RS14860 (J8397_14670) 3109043..3109300 + 258 WP_003899489.1 hypothetical protein -
J8397_RS14865 (J8397_14675) 3109405..3109716 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3094346..3118512 24166


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T198850 WP_003414166.1 NZ_CP072790:c3104667-3104311 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T198850 NZ_CP097031:2656085-2656189 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT198850 WP_003901465.1 NZ_CP072790:c3104881-3104651 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT198850 NZ_CP097031:c2655945-2655791 [Enterococcus faecalis]
TTTTTAGGTAAGTGTGCTATAATAGCAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGTGGTTTAAGA
CGGTGACCTTTTGGATTATTTAAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCTTCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References