Detailed information of TA system TA198849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3064727..3065414
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: J8397_RS14600
• Protein ID: WP_003414064.1
• Coordinates: 3065019..3065414 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: J8397_RS14595
• Protein ID: WP_003414061.1
• Coordinates: 3064727..3064993 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS14565 (J8397_14390)	3060366..3061268	-	903	WP_075911274.1	4-hydroxy-tetrahydrodipicolinate synthase	-
J8397_RS14570 (J8397_14395)	3061337..3062089	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
J8397_RS14575	3062116..3062253	-	138	Protein_2876	type II toxin-antitoxin system VapC family toxin	-
J8397_RS14580 (J8397_14400)	3062333..3062608	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
J8397_RS14585 (J8397_14405)	3062605..3064227	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
J8397_RS14590 (J8397_14410)	3064314..3064730	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
J8397_RS14595 (J8397_14415)	3064727..3064993	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8397_RS14600 (J8397_14420)	3065019..3065414	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8397_RS14605 (J8397_14425)	3065411..3065680	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
J8397_RS14610 (J8397_14430)	3065690..3066784	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
J8397_RS14615 (J8397_14435)	3066781..3067200	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
J8397_RS14620	3067199..3067273	+	75	Protein_2885	hypothetical protein	-
J8397_RS14625 (J8397_14440)	3067274..3067753	-	480	WP_003414073.1	dihydrofolate reductase	-
J8397_RS14630 (J8397_14445)	3067824..3068624	-	801	WP_003911953.1	thymidylate synthase	-
J8397_RS14635 (J8397_14450)	3068780..3069517	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T198849 WP_003414064.1 NZ_CP072790:c3065414-3065019 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T198849 NZ_CP097031:c2583483-2583340 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTACTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT198849 WP_003414061.1 NZ_CP072790:c3064993-3064727 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT198849 NZ_CP097031:2583584-2583733 [Enterococcus faecalis]
TTTTTAGGGAAATGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCGATTGTATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATT

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64