Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2865124..2865761 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag J8397_RS13500 Protein ID WP_003413180.1
Coordinates 2865124..2865519 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag J8397_RS13505 Protein ID WP_003413183.1
Coordinates 2865516..2865761 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS13460 (J8397_13285) 2860527..2861738 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
J8397_RS13465 (J8397_13290) 2861865..2862524 + 660 WP_003900846.1 LppA family lipoprotein -
J8397_RS13470 (J8397_13295) 2862521..2863183 + 663 WP_003900848.1 LppA family lipoprotein -
J8397_RS13475 (J8397_13300) 2863180..2863458 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
J8397_RS13480 (J8397_13305) 2863551..2863964 + 414 WP_003912787.1 PIN domain nuclease -
J8397_RS13485 (J8397_13310) 2864003..2864260 + 258 WP_003413167.1 CopG family transcriptional regulator -
J8397_RS13490 (J8397_13315) 2864257..2864634 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
J8397_RS13495 (J8397_13320) 2864650..2865024 - 375 WP_003413177.1 hypothetical protein -
J8397_RS13500 (J8397_13325) 2865124..2865519 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
J8397_RS13505 (J8397_13330) 2865516..2865761 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
J8397_RS13510 (J8397_13335) 2866172..2866591 - 420 WP_003413190.1 A24 family peptidase -
J8397_RS13515 (J8397_13340) 2866603..2867412 - 810 WP_003413193.1 shikimate dehydrogenase -
J8397_RS13520 (J8397_13345) 2867409..2868662 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
J8397_RS13525 (J8397_13350) 2868655..2869167 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T198845 WP_003413180.1 NZ_CP072790:c2865519-2865124 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T198845 NZ_CP097031:462574-462690 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGTTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT198845 WP_003413183.1 NZ_CP072790:c2865761-2865516 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT198845 NZ_CP097031:c462806-462601 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAA
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References