Detailed information of TA system TA198842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2849664..2850304
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: J8397_RS13395
• Protein ID: WP_003412970.1
• Coordinates: 2849664..2850083 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: J8397_RS13400
• Protein ID: WP_003412975.1
• Coordinates: 2850080..2850304 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS13365 (J8397_13190)	2845249..2845971	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
J8397_RS13370 (J8397_13195)	2846488..2846715	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
J8397_RS13375 (J8397_13200)	2846712..2847113	+	402	WP_003412963.1	PIN domain-containing protein	-
J8397_RS13380 (J8397_13205)	2847148..2848068	-	921	WP_003412965.1	restriction endonuclease	-
J8397_RS13385 (J8397_13210)	2848409..2848654	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
J8397_RS13390 (J8397_13215)	2848713..2849663	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
J8397_RS13395 (J8397_13220)	2849664..2850083	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8397_RS13400 (J8397_13225)	2850080..2850304	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
J8397_RS13405 (J8397_13230)	2850335..2853178	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
J8397_RS13410 (J8397_13235)	2853250..2853651	-	402	WP_003412981.1	hypothetical protein	-
J8397_RS13415 (J8397_13240)	2853651..2854121	-	471	WP_003899364.1	transcription antitermination factor NusB	-
J8397_RS13420 (J8397_13245)	2854124..2854687	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T198842 WP_003412970.1 NZ_CP072790:c2850083-2849664 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T198842 NZ_CP097030:2681978-2682121 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT198842 WP_003412975.1 NZ_CP072790:c2850304-2850080 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT198842 NZ_CP097030:c2681881-2681680 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTGTAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09