Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2321262..2321898 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag J8397_RS10900 Protein ID WP_003410654.1
Coordinates 2321488..2321898 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag J8397_RS10895 Protein ID WP_003410651.1
Coordinates 2321262..2321495 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS10880 2316719..2317111 + 393 WP_229303073.1 metal ABC transporter permease -
J8397_RS10885 (J8397_10745) 2317112..2317516 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
J8397_RS10890 (J8397_10750) 2317600..2321184 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
J8397_RS10895 (J8397_10755) 2321262..2321495 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
J8397_RS10900 (J8397_10760) 2321488..2321898 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
J8397_RS10905 (J8397_10765) 2321882..2322973 + 1092 WP_003900454.1 precorrin-3B synthase -
J8397_RS10910 (J8397_10770) 2322983..2323609 + 627 WP_003410658.1 precorrin-8X methylmutase -
J8397_RS10915 (J8397_10775) 2323606..2325132 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
J8397_RS10920 (J8397_10780) 2325078..2326301 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T198838 WP_003410654.1 NZ_CP072790:2321488-2321898 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T198838 NZ_CP097030:2681291-2681488 [Enterococcus faecalis]
ATGTGTAGAGAAGTTATGGCGGTGGCTATCCAATCTGAAAGAGAGGTGATGCGTATGCACGTATTTCCGAAATTTAGAGA
AAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGGTATGTTTACCATTGCTTTGATTGCGC
TGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT198838 WP_003410651.1 NZ_CP072790:2321262-2321495 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT198838 NZ_CP097030:c2681234-2681171 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References