Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2253476..2254102 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag J8397_RS10610 Protein ID WP_003410075.1
Coordinates 2253704..2254102 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag J8397_RS10605 Protein ID WP_003911750.1
Coordinates 2253476..2253703 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS10595 (J8397_10460) 2251515..2251859 - 345 WP_003410065.1 ferredoxin family protein -
J8397_RS10600 (J8397_10465) 2252048..2253217 - 1170 WP_003899126.1 ATP-binding protein -
J8397_RS10605 (J8397_10470) 2253476..2253703 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J8397_RS10610 (J8397_10475) 2253704..2254102 + 399 WP_003410075.1 PIN domain nuclease Toxin
J8397_RS10615 (J8397_10480) 2254285..2254716 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
J8397_RS10620 (J8397_10485) 2254817..2255251 + 435 WP_003914358.1 DUF1398 domain-containing protein -
J8397_RS10625 (J8397_10490) 2255688..2255867 - 180 Protein_2098 hypothetical protein -
J8397_RS10630 (J8397_10495) 2255955..2256575 + 621 WP_003410086.1 IS110 family transposase -
J8397_RS10635 (J8397_10500) 2256529..2257119 + 591 WP_003899131.1 IS110 family transposase -
J8397_RS10640 (J8397_10505) 2257247..2258503 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T198836 WP_003410075.1 NZ_CP072790:2253704-2254102 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T198836 NZ_CP097030:c2674564-2674223 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAGTCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT198836 WP_003911750.1 NZ_CP072790:2253476-2253703 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT198836 NZ_CP097030:c2674794-2674564 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References