Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2229736..2230322 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag J8397_RS10505 Protein ID WP_003912702.1
Coordinates 2229736..2230080 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag J8397_RS10510 Protein ID WP_003410014.1
Coordinates 2230074..2230322 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS10470 (J8397_10335) 2225442..2226041 + 600 WP_003409989.1 L-lysine exporter -
J8397_RS10475 (J8397_10340) 2226457..2226885 + 429 WP_003409992.1 cellulose-binding protein -
J8397_RS10480 (J8397_10345) 2227111..2227650 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
J8397_RS10485 (J8397_10350) 2228170..2228730 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
J8397_RS10490 (J8397_10355) 2228727..2229068 - 342 WP_003410003.1 DUF2384 domain-containing protein -
J8397_RS10495 (J8397_10360) 2229154..2229411 + 258 WP_003410006.1 hypothetical protein -
J8397_RS10500 (J8397_10365) 2229312..2229647 - 336 WP_003410009.1 dehydrogenase -
J8397_RS10505 (J8397_10370) 2229736..2230080 - 345 WP_003912702.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
J8397_RS10510 (J8397_10375) 2230074..2230322 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
J8397_RS10515 (J8397_10380) 2230422..2232737 - 2316 WP_003899120.1 cation transporter ATPase CptG -
J8397_RS10520 (J8397_10385) 2232734..2233006 - 273 WP_003410017.1 DUF1490 family protein -
J8397_RS10525 (J8397_10390) 2233059..2233415 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
J8397_RS10530 (J8397_10395) 2233572..2234339 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12260.15 Da        Isoelectric Point: 9.8887

>T198835 WP_003912702.1 NZ_CP072790:c2230080-2229736 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPSNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T198835 NZ_CP097030:c2586754-2586611 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTTCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT198835 WP_003410014.1 NZ_CP072790:c2230322-2230074 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT198835 NZ_CP097030:2586494-2586678 [Enterococcus faecalis]
TGCTAGAATGTAGATGAAAAGAGAGAGATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTGAGACGGTGACCAATTGT
ATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGAAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References