Detailed information of TA system TA198834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2228170..2229068
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: J8397_RS10485
• Protein ID: WP_003410001.1
• Coordinates: 2228170..2228730 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: J8397_RS10490
• Protein ID: WP_003410003.1
• Coordinates: 2228727..2229068 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS10455 (J8397_10320)	2223339..2223992	-	654	WP_003409976.1	cutinase Cfp21	-
J8397_RS10460 (J8397_10325)	2224107..2224337	+	231	WP_003409980.1	DUF167 domain-containing protein	-
J8397_RS10465 (J8397_10330)	2224422..2225333	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
J8397_RS10470 (J8397_10335)	2225442..2226041	+	600	WP_003409989.1	L-lysine exporter	-
J8397_RS10475 (J8397_10340)	2226457..2226885	+	429	WP_003409992.1	cellulose-binding protein	-
J8397_RS10480 (J8397_10345)	2227111..2227650	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
J8397_RS10485 (J8397_10350)	2228170..2228730	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
J8397_RS10490 (J8397_10355)	2228727..2229068	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
J8397_RS10495 (J8397_10360)	2229154..2229411	+	258	WP_003410006.1	hypothetical protein	-
J8397_RS10500 (J8397_10365)	2229312..2229647	-	336	WP_003410009.1	dehydrogenase	-
J8397_RS10505 (J8397_10370)	2229736..2230080	-	345	WP_003912702.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
J8397_RS10510 (J8397_10375)	2230074..2230322	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
J8397_RS10515 (J8397_10380)	2230422..2232737	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
J8397_RS10520 (J8397_10385)	2232734..2233006	-	273	WP_003410017.1	DUF1490 family protein	-
J8397_RS10525 (J8397_10390)	2233059..2233415	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T198834 WP_003410001.1 NZ_CP072790:c2228730-2228170 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T198834 NZ_CP097030:506435-506551 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT198834 WP_003410003.1 NZ_CP072790:c2229068-2228727 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT198834 NZ_CP097030:c506667-506462 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• PDB: 6FKG

Antitoxin

• PDB: 6FKG
• AlphaFold DB: A0A7U4FAE4