Detailed information of TA system TA198830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2190074..2190777
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: J8397_RS10230
• Protein ID: WP_003409778.1
• Coordinates: 2190074..2190403 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: J8397_RS10235
• Protein ID: WP_003409780.1
• Coordinates: 2190400..2190777 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS10210 (J8397_10075)	2186457..2187527	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
J8397_RS10215 (J8397_10080)	2187524..2188039	+	516	WP_003409718.1	flavin reductase family protein	-
J8397_RS10220 (J8397_10085)	2188036..2189097	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
J8397_RS10225 (J8397_10090)	2189094..2189864	+	771	WP_003409775.1	SDR family oxidoreductase	-
J8397_RS10230 (J8397_10095)	2190074..2190403	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J8397_RS10235 (J8397_10100)	2190400..2190777	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
J8397_RS10240 (J8397_10105)	2190774..2191364	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
J8397_RS10245 (J8397_10110)	2191419..2192783	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
J8397_RS10250 (J8397_10115)	2192938..2193390	-	453	WP_003899095.1	lipoprotein	-
J8397_RS10255 (J8397_10120)	2193454..2193855	+	402	WP_003409869.1	hypothetical protein	-
J8397_RS10260 (J8397_10125)	2193848..2194030	-	183	WP_003409870.1	hypothetical protein	-
J8397_RS10265 (J8397_10130)	2194144..2194494	-	351	WP_003899096.1	hypothetical protein	-
J8397_RS10270 (J8397_10135)	2194505..2195407	-	903	WP_003899097.1	hypothetical protein	-
J8397_RS10275 (J8397_10140)	2195428..2195619	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T198830 WP_003409778.1 NZ_CP072790:c2190403-2190074 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T198830 NZ_CP097025:16478-16735 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT198830 WP_003409780.1 NZ_CP072790:c2190777-2190400 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT198830 NZ_CP097025:16216-16485 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8