Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1953851..1954464 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag J8397_RS09155 Protein ID WP_003408465.1
Coordinates 1953851..1954240 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag J8397_RS09160 Protein ID WP_003408469.1
Coordinates 1954237..1954464 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS09120 (J8397_08985) 1949480..1950394 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
J8397_RS09125 (J8397_08990) 1950397..1951227 + 831 WP_003408448.1 cyclase family protein -
J8397_RS09130 (J8397_08995) 1951227..1951577 + 351 WP_003898986.1 cupin domain-containing protein -
J8397_RS09135 (J8397_09000) 1951630..1952448 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
J8397_RS09140 (J8397_09005) 1952462..1953241 + 780 WP_070892774.1 IclR family transcriptional regulator -
J8397_RS09150 1953672..1953803 + 132 Protein_1803 IS3 family transposase -
J8397_RS09155 (J8397_09015) 1953851..1954240 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
J8397_RS09160 (J8397_09020) 1954237..1954464 - 228 WP_003408469.1 antitoxin Antitoxin
J8397_RS09165 (J8397_09025) 1954682..1956166 + 1485 WP_003408473.1 biotin carboxylase -
J8397_RS09170 (J8397_09030) 1956163..1957410 + 1248 WP_003408476.1 serine hydrolase -
J8397_RS09175 (J8397_09035) 1957453..1957872 - 420 WP_003408483.1 hypothetical protein -
J8397_RS09180 (J8397_09040) 1957862..1958572 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T198828 WP_003408465.1 NZ_CP072790:c1954240-1953851 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T198828 NZ_CP097022:468394-468735 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT198828 WP_003408469.1 NZ_CP072790:c1954464-1954237 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT198828 NZ_CP097022:468164-468394 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References