Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1771523..1772136 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag J8397_RS08335 Protein ID WP_003407786.1
Coordinates 1771732..1772136 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag J8397_RS08330 Protein ID WP_009935474.1
Coordinates 1771523..1771726 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS08300 (J8397_08170) 1766928..1767308 + 381 WP_003407768.1 fumarate reductase subunit C -
J8397_RS08305 (J8397_08175) 1767305..1767682 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
J8397_RS08310 (J8397_08180) 1767750..1768358 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
J8397_RS08315 (J8397_08185) 1768536..1769690 + 1155 Protein_1638 MMPL family transporter -
J8397_RS08320 (J8397_08190) 1769700..1770146 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
J8397_RS08325 (J8397_08195) 1770181..1771470 + 1290 WP_003407781.1 threonine ammonia-lyase -
J8397_RS08330 (J8397_08200) 1771523..1771726 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J8397_RS08335 (J8397_08205) 1771732..1772136 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
J8397_RS08340 (J8397_08210) 1772153..1773895 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
J8397_RS08345 (J8397_08215) 1773888..1776185 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T198827 WP_003407786.1 NZ_CP072790:1771732-1772136 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T198827 NZ_CP097022:181478-181744 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT198827 WP_009935474.1 NZ_CP072790:1771523-1771726 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT198827 NZ_CP097022:181215-181478 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References