Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1580568..1581223 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA6
Locus tag J8397_RS07490 Protein ID WP_003898861.1
Coordinates 1580568..1580969 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag J8397_RS07495 Protein ID WP_003407272.1
Coordinates 1580966..1581223 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS07475 (J8397_07335) 1576040..1577425 - 1386 WP_003407260.1 cytochrome P450 -
J8397_RS07480 (J8397_07340) 1577503..1578537 + 1035 WP_003407264.1 AraC family transcriptional regulator -
J8397_RS07485 (J8397_07345) 1578583..1580313 - 1731 WP_031694300.1 PE family protein -
J8397_RS07490 (J8397_07350) 1580568..1580969 - 402 WP_003898861.1 type II toxin-antitoxin system VapC family toxin Toxin
J8397_RS07495 (J8397_07355) 1580966..1581223 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
J8397_RS07500 (J8397_07360) 1581306..1582265 - 960 WP_003407276.1 alpha/beta hydrolase -
J8397_RS07505 (J8397_07365) 1582290..1583252 - 963 WP_003407279.1 alpha/beta hydrolase -
J8397_RS07510 (J8397_07370) 1583251..1583988 + 738 WP_031647000.1 lysoplasmalogenase -
J8397_RS07515 (J8397_07375) 1584069..1586036 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T198825 WP_003898861.1 NZ_CP072790:c1580969-1580568 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T198825 NZ_CP097021:c42346-42005 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT198825 WP_003407272.1 NZ_CP072790:c1581223-1580966 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT198825 NZ_CP097021:c42576-42346 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045ISB0

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References