Detailed information of TA system TA198824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1546198..1547248
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: -
• Locus tag: J8397_RS07340
• Protein ID: WP_003912801.1
• Coordinates: 1546198..1547019 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: J8397_RS07345
• Protein ID: WP_003407167.1
• Coordinates: 1547012..1547248 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS07320 (J8397_07180)	1541756..1542418	-	663	WP_003407152.1	membrane protein	-
J8397_RS07325 (J8397_07185)	1542415..1543200	-	786	WP_003407157.1	membrane protein	-
J8397_RS07330 (J8397_07190)	1543491..1545452	-	1962	WP_003407159.1	sigma-F factor regulator	-
J8397_RS07335 (J8397_07195)	1545591..1545977	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
J8397_RS07340 (J8397_07200)	1546198..1547019	+	822	WP_003912801.1	GTP pyrophosphokinase family protein	Toxin
J8397_RS07345 (J8397_07205)	1547012..1547248	+	237	WP_003407167.1	hypothetical protein	Antitoxin
J8397_RS07350 (J8397_07210)	1547320..1548525	-	1206	WP_003898846.1	serine hydrolase domain-containing protein	-
J8397_RS07355 (J8397_07215)	1548828..1549613	+	786	WP_003407180.1	lipoprotein LprF	-
J8397_RS07360 (J8397_07220)	1549809..1551278	+	1470	WP_003900326.1	cytochrome b5 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30134.12 Da
• Isoelectric Point: 5.8187

>T198824 WP_003912801.1 NZ_CP072790:1546198-1547019 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADFLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T198824 NZ_CP097020:2662712-2662855 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAACCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT198824 WP_003407167.1 NZ_CP072790:1547012-1547248 [Mycobacterium tuberculosis]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT198824 NZ_CP097020:c2663049-2662787 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BTV8