Detailed information of TA system TA198822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1390411..1391099
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: J8397_RS06655
• Protein ID: WP_003406304.1
• Coordinates: 1390668..1391099 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: J8397_RS06650
• Protein ID: WP_003406302.1
• Coordinates: 1390411..1390671 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS06630 (J8397_06500)	1385988..1386812	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
J8397_RS06635 (J8397_06505)	1386817..1387998	+	1182	WP_070892757.1	trehalose ABC transporter ATP-binding protein SugC	-
J8397_RS06640 (J8397_06510)	1388075..1389175	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
J8397_RS06645 (J8397_06515)	1389346..1390335	+	990	WP_003406301.1	malate dehydrogenase	-
J8397_RS06650 (J8397_06520)	1390411..1390671	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
J8397_RS06655 (J8397_06525)	1390668..1391099	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8397_RS06660 (J8397_06530)	1391122..1392810	-	1689	WP_003910308.1	PE family protein	-
J8397_RS06665 (J8397_06535)	1392990..1393850	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
J8397_RS06670 (J8397_06540)	1393931..1394761	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
J8397_RS06675 (J8397_06545)	1394818..1395111	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
J8397_RS06680 (J8397_06550)	1395108..1395377	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T198822 WP_003406304.1 NZ_CP072790:1390668-1391099 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T198822 NZ_CP097020:2662332-2662481 [Enterococcus faecalis]
ATGCGTATGCACGTATTTCCGAAATTCAGAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGG
GTTTGGTATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT198822 WP_003406302.1 NZ_CP072790:1390411-1390671 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT198822 NZ_CP097020:c2662615-2662414 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6