Detailed information of TA system TA198821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1245835..1246403
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: J8397_RS05985
• Protein ID: WP_003405865.1
• Coordinates: 1246029..1246403 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: J8397_RS05980
• Protein ID: WP_003405863.1
• Coordinates: 1245835..1246032 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS05960 (J8397_05855)	1241876..1242514	-	639	WP_003898730.1	lipid droplet-associated protein	-
J8397_RS05965 (J8397_05860)	1242604..1243611	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
J8397_RS05970 (J8397_05865)	1243628..1244611	-	984	WP_003898731.1	hypothetical protein	-
J8397_RS05975 (J8397_05870)	1244674..1245747	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
J8397_RS05980 (J8397_05875)	1245835..1246032	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8397_RS05985 (J8397_05880)	1246029..1246403	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8397_RS05990 (J8397_05885)	1246606..1247304	+	699	WP_003898733.1	hypothetical protein	-
J8397_RS05995 (J8397_05890)	1247422..1247607	+	186	WP_003901093.1	hypothetical protein	-
J8397_RS06000 (J8397_05895)	1247534..1247809	-	276	WP_003405867.1	hypothetical protein	-
J8397_RS06005 (J8397_05900)	1248052..1248375	+	324	WP_003405871.1	putative quinol monooxygenase	-
J8397_RS06010 (J8397_05905)	1248390..1249088	-	699	WP_031646953.1	hypothetical protein	-
J8397_RS06015 (J8397_05910)	1249122..1249331	+	210	WP_003911400.1	hypothetical protein	-
J8397_RS06020 (J8397_05915)	1249283..1250053	-	771	Protein_1185	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T198821 WP_003405865.1 NZ_CP072790:1246029-1246403 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T198821 NZ_CP097020:c2656905-2656564 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
TTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGCAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT198821 WP_003405863.1 NZ_CP072790:1245835-1246032 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT198821 NZ_CP097020:c2657135-2656905 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07