Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 760018..760810 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag J8397_RS03520 Protein ID WP_003403386.1
Coordinates 760373..760810 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag J8397_RS03515 Protein ID WP_003403381.1
Coordinates 760018..760263 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS03485 (J8397_03445) 755038..756543 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
J8397_RS03490 (J8397_03450) 756624..757634 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
J8397_RS03495 (J8397_03455) 758022..758405 - 384 WP_003912919.1 ribonuclease VapC6 -
J8397_RS03500 (J8397_03460) 758500..758655 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
J8397_RS03505 (J8397_03465) 758731..759447 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
J8397_RS03510 (J8397_03470) 759723..760031 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
J8397_RS03515 (J8397_03475) 760018..760263 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
J8397_RS03520 (J8397_03480) 760373..760810 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
J8397_RS03525 (J8397_03485) 760807..761061 - 255 WP_003911263.1 antitoxin VapB7 -
J8397_RS03530 (J8397_03490) 761175..763538 + 2364 WP_003898547.1 arylsulfatase AtsD -
J8397_RS03535 (J8397_03495) 763601..763927 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8397_RS03540 (J8397_03500) 763839..764177 + 339 WP_003403405.1 PIN domain-containing protein -
J8397_RS03545 (J8397_03505) 764174..764347 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T198814 WP_003403386.1 NZ_CP072790:c760810-760373 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T198814 NZ_CP097020:c308528-308433 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT198814 WP_003403381.1 NZ_CP072790:c760263-760018 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT198814 NZ_CP097020:308334-308398 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References