Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 722530..723194 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID P96917
Locus tag J8397_RS03325 Protein ID WP_003403246.1
Coordinates 722787..723194 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF18
Locus tag J8397_RS03320 Protein ID WP_003403244.1
Coordinates 722530..722790 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS03295 (J8397_03255) 718707..719771 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
J8397_RS03300 (J8397_03260) 719875..720822 + 948 WP_003403232.1 DUF732 domain-containing protein -
J8397_RS03305 (J8397_03265) 720915..721169 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 -
J8397_RS03310 (J8397_03270) 721169..721564 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 -
J8397_RS03315 (J8397_03275) 721658..722398 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
J8397_RS03320 (J8397_03280) 722530..722790 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J8397_RS03325 (J8397_03285) 722787..723194 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin Toxin
J8397_RS03330 (J8397_03290) 723266..724417 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
J8397_RS03335 (J8397_03295) 724510..726237 - 1728 WP_070892740.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-57)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14376.37 Da        Isoelectric Point: 4.3568

>T198811 WP_003403246.1 NZ_CP072790:722787-723194 [Mycobacterium tuberculosis]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Download         Length: 408 bp

>T198811 NZ_CP097013:32928-33272 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTACACAAAAAGGCTTGTTTCGA
ACCAAAGTATGGTGTAATTTTCATTAATCAAGATTTATCTACTGCAGAACAAGAAGAAGCTATTTATCATGAATTTAAGC
ACGTGAAAGATCATGCTGATTTGATGGCACTATACAACATTCCTATTTTCAGATCAAAAATGGAAGCAGAAGCTGAACAT
TATATGCTCGAATGTTTAATCGAAAAGAACGGTGGTCAATTTAACTATTCTAATGTAATCACACATTATAATTTAAAGAT
GGGCCAAGAAATTTACTTAAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9492.84 Da        Isoelectric Point: 11.9108

>AT198811 WP_003403244.1 NZ_CP072790:722530-722790 [Mycobacterium tuberculosis]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT198811 NZ_CP097013:32587-32910 [Enterococcus faecalis]
ATGACTGTATTTGATAGAGTTAAAAAATTAGCAGATAGTCAGAAAATATCTATTGTGGAACTTGAAGAAAAGTTAAATTT
TAGTCGAAATTCATTGTATGCTTGGAAAAAAAGCAAGCCTTCTATCGATAAATTAGAAGCTGTCGCAAATTATTTTGGAG
TTTCAACGGATTATTTATTGGGTCGTGAAATCTCTAATAAACCAAAGCAATCTGATGATTTAGATGAAGTATTAGACAAT
GTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAGATTCGGAAA
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3DBO

Antitoxin

Source ID Structure
PDB 3DBO
AlphaFold DB A0A7U4BSE4

References