Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 699464..700110 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag J8397_RS03155 Protein ID WP_003403122.1
Coordinates 699464..699856 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag J8397_RS03160 Protein ID WP_003403125.1
Coordinates 699853..700110 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS03140 (J8397_03100) 695126..696652 + 1527 WP_003403112.1 virulence factor Mce family protein -
J8397_RS03145 (J8397_03105) 696715..697857 + 1143 WP_003911253.1 virulence factor Mce family protein -
J8397_RS03150 (J8397_03110) 697862..699412 + 1551 WP_003403119.1 MlaD family protein -
J8397_RS03155 (J8397_03115) 699464..699856 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
J8397_RS03160 (J8397_03120) 699853..700110 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
J8397_RS03165 (J8397_03125) 700293..701528 - 1236 WP_003403128.1 ATP-binding protein -
J8397_RS03170 (J8397_03130) 701779..702192 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
J8397_RS03175 (J8397_03135) 702189..702425 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
J8397_RS03180 (J8397_03140) 702529..703035 - 507 WP_003900973.1 two component system sensor kinase HK1 -
J8397_RS03185 (J8397_03145) 703149..703559 - 411 WP_070892737.1 HAMP domain-containing protein -
J8397_RS03190 (J8397_03150) 703543..704238 - 696 WP_206647908.1 response regulator transcription factor -
J8397_RS03195 (J8397_03155) 704361..704672 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T198806 WP_003403122.1 NZ_CP072790:c699856-699464 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T198806 NZ_CP097010:912258-912401 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT198806 WP_003403125.1 NZ_CP072790:c700110-699853 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT198806 NZ_CP097010:c912595-912333 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATA
ATAGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References