Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3831377..3831960 Replicon chromosome
Accession NZ_CP072787
Organism Escherichia coli strain NC101

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I0P59_RS18175 Protein ID WP_000254750.1
Coordinates 3831377..3831712 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I0P59_RS18180 Protein ID WP_000581937.1
Coordinates 3831712..3831960 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0P59_RS18160 (3827263) 3827263..3828561 - 1299 WP_000036723.1 phosphopyruvate hydratase -
I0P59_RS18165 (3828649) 3828649..3830286 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
I0P59_RS18170 (3830514) 3830514..3831305 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
I0P59_RS18175 (3831377) 3831377..3831712 - 336 WP_000254750.1 endoribonuclease MazF Toxin
I0P59_RS18180 (3831712) 3831712..3831960 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I0P59_RS18185 (3832038) 3832038..3834272 - 2235 WP_000226815.1 GTP pyrophosphokinase -
I0P59_RS18190 (3834320) 3834320..3835621 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T198793 WP_000254750.1 NZ_CP072787:c3831712-3831377 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T198793 NZ_CP097008:495003-495119 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT198793 WP_000581937.1 NZ_CP072787:c3831960-3831712 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT198793 NZ_CP097008:c495235-495030 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References