Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1377213..1377831 Replicon chromosome
Accession NZ_CP072787
Organism Escherichia coli strain NC101

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I0P59_RS06440 Protein ID WP_001291435.1
Coordinates 1377213..1377431 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I0P59_RS06445 Protein ID WP_000344800.1
Coordinates 1377457..1377831 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0P59_RS06405 (1372501) 1372501..1373073 + 573 WP_000779831.1 YbaY family lipoprotein -
I0P59_RS06410 (1373103) 1373103..1373414 - 312 WP_000409908.1 MGMT family protein -
I0P59_RS06420 (1373793) 1373793..1374146 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I0P59_RS06425 (1374188) 1374188..1375738 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I0P59_RS06430 (1375902) 1375902..1376372 - 471 WP_000136192.1 YlaC family protein -
I0P59_RS06435 (1376488) 1376488..1377039 - 552 WP_000102539.1 maltose O-acetyltransferase -
I0P59_RS06440 (1377213) 1377213..1377431 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I0P59_RS06445 (1377457) 1377457..1377831 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I0P59_RS06450 (1378376) 1378376..1381525 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I0P59_RS06455 (1381548) 1381548..1382741 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T198780 WP_001291435.1 NZ_CP072787:c1377431-1377213 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T198780 NZ_CP072787:c1377431-1377213 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT198780 WP_000344800.1 NZ_CP072787:c1377831-1377457 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT198780 NZ_CP072787:c1377831-1377457 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References