Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3996960..3997614 Replicon chromosome
Accession NZ_CP072786
Organism Escherichia coli strain JA0257

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I0P57_RS18855 Protein ID WP_000244781.1
Coordinates 3996960..3997367 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I0P57_RS18860 Protein ID WP_000354046.1
Coordinates 3997348..3997614 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0P57_RS18835 (3992917) 3992917..3994650 - 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -
I0P57_RS18840 (3994656) 3994656..3995366 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I0P57_RS18845 (3995391) 3995391..3996287 - 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
I0P57_RS18850 (3996399) 3996399..3996920 + 522 WP_001055874.1 flavodoxin FldB -
I0P57_RS18855 (3996960) 3996960..3997367 - 408 WP_000244781.1 protein YgfX Toxin
I0P57_RS18860 (3997348) 3997348..3997614 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I0P57_RS18865 (3997857) 3997857..3998837 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
I0P57_RS18870 (3998914) 3998914..3999573 - 660 WP_000250274.1 hemolysin III family protein -
I0P57_RS18875 (3999737) 3999737..4000048 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I0P57_RS18880 (4000093) 4000093..4001526 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T198770 WP_000244781.1 NZ_CP072786:c3997367-3996960 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T198770 NZ_CP096976:c5043141-5042830 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT198770 WP_000354046.1 NZ_CP072786:c3997614-3997348 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT198770 NZ_CP096976:c5042829-5042539 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References