Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 470218..470715 Replicon chromosome
Accession NZ_CP072784
Organism Escherichia coli strain JA0266

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I0P58_RS02155 Protein ID WP_001173343.1
Coordinates 470542..470715 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I0P58_RS02150 Protein ID WP_001312331.1
Coordinates 470218..470514 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0P58_RS02130 (466946) 466946..467665 - 720 WP_000611288.1 two-component system response regulator DcuR -
I0P58_RS02135 (467662) 467662..469293 - 1632 WP_001216466.1 sensor histidine kinase -
I0P58_RS02140 (469474) 469474..469704 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
I0P58_RS02145 (469716) 469716..469988 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I0P58_RS02150 (470218) 470218..470514 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I0P58_RS02155 (470542) 470542..470715 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I0P58_RS02160 (470834) 470834..472351 - 1518 WP_000003805.1 lysine--tRNA ligase -
I0P58_RS02165 (472588) 472588..474045 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198702 WP_001173343.1 NZ_CP072784:470542-470715 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198702 NZ_CP096958:c179586-179305 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGATAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT198702 WP_001312331.1 NZ_CP072784:470218-470514 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198702 NZ_CP096958:c179810-179583 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References