Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4938421..4939029 Replicon chromosome
Accession NZ_CP072783
Organism Pseudomonas aeruginosa strain LICME WGH-6

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag J9247_RS23050 Protein ID WP_019486378.1
Coordinates 4938421..4938768 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag J9247_RS23055 Protein ID WP_003114155.1
Coordinates 4938778..4939029 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9247_RS23020 (J9247_23020) 4933840..4934052 + 213 WP_033978412.1 cysteine-rich CWC family protein -
J9247_RS23025 (J9247_23025) 4934052..4934744 + 693 WP_033972501.1 16S rRNA pseudouridine(516) synthase -
J9247_RS23030 (J9247_23030) 4934880..4935923 + 1044 WP_033972500.1 L,D-transpeptidase -
J9247_RS23035 (J9247_23035) 4936003..4936740 + 738 WP_039027227.1 murein L,D-transpeptidase catalytic domain family protein -
J9247_RS23040 (J9247_23040) 4937192..4938094 + 903 WP_083556998.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
J9247_RS23050 (J9247_23050) 4938421..4938768 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J9247_RS23055 (J9247_23055) 4938778..4939029 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J9247_RS23060 (J9247_23060) 4939243..4940226 - 984 WP_019682343.1 tyrosine-type recombinase/integrase -
J9247_RS23065 (J9247_23065) 4940226..4941518 - 1293 WP_058144024.1 hypothetical protein -
J9247_RS23070 (J9247_23070) 4941748..4943022 - 1275 WP_128667640.1 zonular occludens toxin family protein -
J9247_RS23075 (J9247_23075) 4943026..4943382 - 357 WP_003114150.1 DUF2523 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4938421..4959664 21243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T198699 WP_019486378.1 NZ_CP072783:c4938768-4938421 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T198699 NZ_CP096956:c5547383-5547105 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT198699 WP_003114155.1 NZ_CP072783:c4939029-4938778 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT198699 NZ_CP096956:c5547093-5546788 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References