Detailed information of TA system TA198693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3792659..3793308
• Replicon: chromosome
• Accession: NZ_CP072779
• Organism: Proteus mirabilis strain 1035

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: J8N15_RS17615
• Protein ID: WP_012368534.1
• Coordinates: 3792659..3793078 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: J8N15_RS17620
• Protein ID: WP_012368535.1
• Coordinates: 3793075..3793308 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8N15_RS17585 (J8N15_17585)	3787938..3788816	-	879	WP_017628653.1	23S rRNA pseudouridine(2604) synthase RluF	-
J8N15_RS17590 (J8N15_17590)	3788992..3789906	-	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
J8N15_RS17595 (J8N15_17595)	3789930..3790367	-	438	WP_004246810.1	D-aminoacyl-tRNA deacylase	-
J8N15_RS17600 (J8N15_17600)	3790448..3791065	-	618	WP_004246809.1	glucose-1-phosphatase	-
J8N15_RS17605 (J8N15_17605)	3791717..3792133	-	417	WP_046334846.1	hypothetical protein	-
J8N15_RS17610 (J8N15_17610)	3792111..3792434	-	324	WP_104731870.1	hypothetical protein	-
J8N15_RS17615 (J8N15_17615)	3792659..3793078	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8N15_RS17620 (J8N15_17620)	3793075..3793308	-	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8N15_RS17625 (J8N15_17625)	3793575..3793727	+	153	WP_172764486.1	hypothetical protein	-
J8N15_RS17630 (J8N15_17630)	3794689..3795897	+	1209	WP_060555750.1	DUF262 domain-containing protein	-
J8N15_RS17635 (J8N15_17635)	3796114..3796335	-	222	WP_060555751.1	hypothetical protein	-
J8N15_RS17640 (J8N15_17640)	3796383..3796670	+	288	WP_104731871.1	transposase	-
J8N15_RS17645 (J8N15_17645)	3797119..3797598	+	480	WP_060555753.1	Hcp family type VI secretion system effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T198693 WP_012368534.1 NZ_CP072779:c3793078-3792659 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T198693 NZ_CP096956:c148558-148277 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT198693 WP_012368535.1 NZ_CP072779:c3793308-3793075 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT198693 NZ_CP096956:c148782-148555 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0