Detailed information of TA system TA198689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2358068..2359046
• Replicon: chromosome
• Accession: NZ_CP072774
• Organism: Bacillus cereus strain BC33

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: J8Y18_RS12180
• Protein ID: WP_000624977.1
• Coordinates: 2358068..2358805 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: J8Y18_RS12185
• Protein ID: WP_000237819.1
• Coordinates: 2358918..2359046 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8Y18_RS12155 (J8Y18_12155)	2353681..2354088	+	408	WP_000072495.1	VOC family protein	-
J8Y18_RS12160 (J8Y18_12160)	2354112..2354492	+	381	Protein_2324	SAM-dependent methyltransferase	-
J8Y18_RS12165 (J8Y18_12165)	2354650..2356326	-	1677	WP_211478120.1	alpha-keto acid decarboxylase family protein	-
J8Y18_RS12170 (J8Y18_12170)	2356443..2356925	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
J8Y18_RS12175 (J8Y18_12175)	2357092..2357829	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
J8Y18_RS12180 (J8Y18_12180)	2358068..2358805	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J8Y18_RS12185 (J8Y18_12185)	2358918..2359046	+	129	WP_000237819.1	hypothetical protein	Antitoxin
J8Y18_RS12190 (J8Y18_12190)	2359119..2359295	+	177	WP_000852629.1	stage II sporulation protein SB	-
J8Y18_RS12195 (J8Y18_12195)	2359311..2359700	-	390	WP_211478121.1	YxeA family protein	-
J8Y18_RS12200 (J8Y18_12200)	2359912..2361381	+	1470	WP_211478122.1	beta-Ala-His dipeptidase	-
J8Y18_RS12205 (J8Y18_12205)	2361627..2362436	+	810	WP_000678369.1	papain-like cysteine protease family protein	-
J8Y18_RS12210 (J8Y18_12210)	2362462..2363064	+	603	WP_000517271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T198689 WP_000624977.1 NZ_CP072774:2358068-2358805 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T198689 NZ_CP096953:1987033-1987620 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGTGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT198689 WP_000237819.1 NZ_CP072774:2358918-2359046 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT198689 NZ_CP096953:1987687-1988541 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGCCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2