Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 236185..236827 Replicon chromosome
Accession NZ_CP072774
Organism Bacillus cereus strain BC33

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag J8Y18_RS01345 Protein ID WP_000635963.1
Coordinates 236477..236827 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag J8Y18_RS01340 Protein ID WP_000004570.1
Coordinates 236185..236472 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8Y18_RS01315 (J8Y18_01315) 231502..232464 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
J8Y18_RS01320 (J8Y18_01320) 232457..233029 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
J8Y18_RS01325 (J8Y18_01325) 233123..233482 + 360 WP_000583416.1 holo-ACP synthase -
J8Y18_RS01330 (J8Y18_01330) 233639..234589 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
J8Y18_RS01335 (J8Y18_01335) 234707..235876 + 1170 WP_000390615.1 alanine racemase -
J8Y18_RS01340 (J8Y18_01340) 236185..236472 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
J8Y18_RS01345 (J8Y18_01345) 236477..236827 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J8Y18_RS01350 (J8Y18_01350) 236895..239063 + 2169 WP_211477896.1 Tex family protein -
J8Y18_RS01355 (J8Y18_01355) 239121..239237 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
J8Y18_RS01360 (J8Y18_01360) 239433..239891 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T198688 WP_000635963.1 NZ_CP072774:236477-236827 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T198688 NZ_CP096953:c179604-179323 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGATAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT198688 WP_000004570.1 NZ_CP072774:236185-236472 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT198688 NZ_CP096953:c179828-179601 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References