Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 259491..260133 Replicon chromosome
Accession NZ_CP072769
Organism Bacillus cereus strain BC07

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag J8Y17_RS01460 Protein ID WP_000635963.1
Coordinates 259783..260133 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag J8Y17_RS01455 Protein ID WP_000004570.1
Coordinates 259491..259778 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8Y17_RS01430 (J8Y17_01430) 254807..255769 + 963 WP_088667741.1 UV DNA damage repair endonuclease UvsE -
J8Y17_RS01435 (J8Y17_01435) 255762..256334 - 573 WP_071728601.1 rhomboid family intramembrane serine protease -
J8Y17_RS01440 (J8Y17_01440) 256427..256786 + 360 WP_211479786.1 holo-ACP synthase -
J8Y17_RS01445 (J8Y17_01445) 256943..257893 + 951 WP_211480096.1 outer membrane lipoprotein carrier protein LolA -
J8Y17_RS01450 (J8Y17_01450) 258012..259181 + 1170 WP_000390607.1 alanine racemase -
J8Y17_RS01455 (J8Y17_01455) 259491..259778 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
J8Y17_RS01460 (J8Y17_01460) 259783..260133 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J8Y17_RS01465 (J8Y17_01465) 260201..262369 + 2169 WP_071728600.1 Tex family protein -
J8Y17_RS01470 (J8Y17_01470) 262427..262543 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
J8Y17_RS01475 (J8Y17_01475) 262739..263197 + 459 WP_071728599.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T198686 WP_000635963.1 NZ_CP072769:259783-260133 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T198686 NZ_CP096951:c149413-148889 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCTCCTGGTCTGCTGATCAGCTATTCCCTCAGTCTGGATCGAGTGATC
GACTTCACCGGCGGCTATAACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAGCCGCCGAGCTGGATTATCGGAGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT198686 WP_000004570.1 NZ_CP072769:259491-259778 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT198686 NZ_CP096951:c149805-149410 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGCGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCGGCTATAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCTTGGGAAGCTGGTGCCCAAGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References