Detailed information of TA system TA198685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2337022..2337997
• Replicon: chromosome
• Accession: NZ_CP072766
• Organism: Bacillus cereus strain BC06

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: J8Y16_RS12055
• Protein ID: WP_061530837.1
• Coordinates: 2337022..2337759 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: J8Y16_RS12060
• Protein ID: WP_061530834.1
• Coordinates: 2337872..2337997 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8Y16_RS12030 (J8Y16_12030)	2332529..2333230	+	702	WP_001991930.1	hypothetical protein	-
J8Y16_RS12035 (J8Y16_12035)	2333357..2334241	+	885	WP_000795514.1	hypothetical protein	-
J8Y16_RS12040 (J8Y16_12040)	2334551..2335339	-	789	Protein_2296	thiamine pyrophosphate-binding protein	-
J8Y16_RS12045 (J8Y16_12045)	2335446..2335928	+	483	WP_001248819.1	MarR family winged helix-turn-helix transcriptional regulator	-
J8Y16_RS12050 (J8Y16_12050)	2336096..2336833	+	738	WP_000594140.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
J8Y16_RS12055 (J8Y16_12055)	2337022..2337759	+	738	WP_061530837.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
J8Y16_RS12060 (J8Y16_12060)	2337872..2337997	+	126	WP_061530834.1	hypothetical protein	Antitoxin
J8Y16_RS12065 (J8Y16_12065)	2338074..2338250	+	177	WP_212805198.1	hypothetical protein	-
J8Y16_RS27215	2338800..2338925	+	126	WP_000694311.1	hypothetical protein	-
J8Y16_RS12075 (J8Y16_12075)	2339000..2339176	+	177	WP_179955797.1	stage II sporulation protein SB	-
J8Y16_RS12080 (J8Y16_12080)	2339320..2340789	+	1470	WP_212805199.1	beta-Ala-His dipeptidase	-
J8Y16_RS12085 (J8Y16_12085)	2341375..2341845	-	471	WP_061530815.1	DUF5065 family protein	-
J8Y16_RS12090 (J8Y16_12090)	2342299..2342958	+	660	WP_212805200.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28361.08 Da
• Isoelectric Point: 8.2671

>T198685 WP_061530837.1 NZ_CP072766:2337022-2337759 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHMEEDYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T198685 NZ_CP096950:c5758638-5758360 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4874.26 Da
• Isoelectric Point: 3.9652

>AT198685 WP_061530834.1 NZ_CP072766:2337872-2337997 [Bacillus cereus]
VIKDNNNDFFKEDNEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT198685 NZ_CP096950:c5758348-5758043 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG