Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 250179..250821 Replicon chromosome
Accession NZ_CP072766
Organism Bacillus cereus strain BC06

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag J8Y16_RS01410 Protein ID WP_000635963.1
Coordinates 250471..250821 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag J8Y16_RS01405 Protein ID WP_000004570.1
Coordinates 250179..250466 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8Y16_RS01380 (J8Y16_01380) 245495..246457 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
J8Y16_RS01385 (J8Y16_01385) 246450..247022 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
J8Y16_RS01390 (J8Y16_01390) 247115..247474 + 360 WP_000635040.1 holo-ACP synthase -
J8Y16_RS01395 (J8Y16_01395) 247631..248581 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
J8Y16_RS01400 (J8Y16_01400) 248700..249869 + 1170 WP_000390600.1 alanine racemase -
J8Y16_RS01405 (J8Y16_01405) 250179..250466 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
J8Y16_RS01410 (J8Y16_01410) 250471..250821 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J8Y16_RS01415 (J8Y16_01415) 250889..253057 + 2169 WP_071737052.1 Tex family protein -
J8Y16_RS01420 (J8Y16_01420) 253115..253231 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
J8Y16_RS01425 (J8Y16_01425) 253427..253885 + 459 WP_000344246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T198684 WP_000635963.1 NZ_CP072766:250471-250821 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T198684 NZ_CP096950:c5532537-5532238 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAATGGAGGCCGCAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT198684 WP_000004570.1 NZ_CP072766:250179-250466 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT198684 NZ_CP096950:c5532241-5531951 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGCGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References