Detailed information of TA system TA198676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3141617..3142221
• Replicon: chromosome
• Accession: NZ_CP072765
• Organism: Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: J8668_RS14840
• Protein ID: WP_003414492.1
• Coordinates: 3141617..3142009 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P71622
• Locus tag: J8668_RS14845
• Protein ID: WP_003912003.1
• Coordinates: 3142006..3142221 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8668_RS14810 (J8668_14810)	3136767..3137555	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
J8668_RS14815 (J8668_14815)	3137889..3138434	-	546	WP_003913758.1	DUF1802 family protein	-
J8668_RS14820 (J8668_14820)	3138706..3139590	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
J8668_RS14825 (J8668_14825)	3139593..3140480	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
J8668_RS14830 (J8668_14830)	3140785..3141330	-	546	WP_003899500.1	DUF1802 family protein	-
J8668_RS14835 (J8668_14835)	3141327..3141596	-	270	WP_003414489.1	DUF2277 family protein	-
J8668_RS14840 (J8668_14840)	3141617..3142009	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8668_RS14845 (J8668_14845)	3142006..3142221	-	216	WP_003912003.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J8668_RS14850 (J8668_14850)	3142268..3143017	+	750	WP_003414497.1	enoyl-CoA hydratase	-
J8668_RS14855 (J8668_14855)	3143096..3144178	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
J8668_RS14860 (J8668_14860)	3144171..3145481	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
J8668_RS14865 (J8668_14865)	3145484..3146311	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
J8668_RS14870 (J8668_14870)	3146308..3147219	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T198676 WP_003414492.1 NZ_CP072765:c3142009-3141617 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T198676 NZ_CP096946:c5816860-5816582 [Pseudomonas aeruginosa]
ATGATTCTGGCCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7483.50 Da
• Isoelectric Point: 4.9732

>AT198676 WP_003912003.1 NZ_CP072765:c3142221-3142006 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT198676 NZ_CP096946:c5816570-5816265 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: P71622