Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3075580..3076267 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag J8668_RS14485 Protein ID WP_003414064.1
Coordinates 3075872..3076267 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag J8668_RS14480 Protein ID WP_003414061.1
Coordinates 3075580..3075846 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS14455 (J8668_14455) 3071219..3072121 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
J8668_RS14460 (J8668_14460) 3072190..3072942 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
J8668_RS20860 3072969..3073106 - 138 Protein_2888 type II toxin-antitoxin system VapC family toxin -
J8668_RS14465 (J8668_14465) 3073186..3073461 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
J8668_RS14470 (J8668_14470) 3073458..3075080 - 1623 WP_003906897.1 class I SAM-dependent DNA methyltransferase -
J8668_RS14475 (J8668_14475) 3075167..3075583 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
J8668_RS14480 (J8668_14480) 3075580..3075846 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J8668_RS14485 (J8668_14485) 3075872..3076267 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
J8668_RS14490 (J8668_14490) 3076264..3076533 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
J8668_RS14495 (J8668_14495) 3076543..3077637 - 1095 WP_003414068.1 restriction endonuclease subunit S -
J8668_RS14500 (J8668_14500) 3077634..3078053 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
J8668_RS20865 3078052..3078126 + 75 Protein_2897 hypothetical protein -
J8668_RS14505 (J8668_14505) 3078127..3078606 - 480 WP_003414073.1 dihydrofolate reductase -
J8668_RS14510 (J8668_14510) 3078677..3079477 - 801 WP_003911953.1 thymidylate synthase -
J8668_RS14515 (J8668_14515) 3079633..3080370 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T198673 WP_003414064.1 NZ_CP072765:c3076267-3075872 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T198673 NZ_CP096946:2025011-2025598 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGTGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT198673 WP_003414061.1 NZ_CP072765:c3075846-3075580 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT198673 NZ_CP096946:2025665-2026519 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGCCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TF65

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References