Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2874724..2875361 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag J8668_RS13390 Protein ID WP_003413180.1
Coordinates 2874724..2875119 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag J8668_RS13395 Protein ID WP_003413183.1
Coordinates 2875116..2875361 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS13350 (J8668_13350) 2870127..2871338 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
J8668_RS13355 (J8668_13355) 2871465..2872124 + 660 WP_003900846.1 LppA family lipoprotein -
J8668_RS13360 (J8668_13360) 2872121..2872783 + 663 WP_003900848.1 LppA family lipoprotein -
J8668_RS13365 (J8668_13365) 2872780..2873058 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
J8668_RS13370 (J8668_13370) 2873151..2873564 + 414 WP_003413164.1 PIN domain nuclease -
J8668_RS13375 (J8668_13375) 2873603..2873860 + 258 WP_003413167.1 CopG family transcriptional regulator -
J8668_RS13380 (J8668_13380) 2873857..2874234 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
J8668_RS13385 (J8668_13385) 2874250..2874624 - 375 WP_003413177.1 hypothetical protein -
J8668_RS13390 (J8668_13390) 2874724..2875119 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
J8668_RS13395 (J8668_13395) 2875116..2875361 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
J8668_RS13400 (J8668_13400) 2875772..2876191 - 420 WP_003413190.1 A24 family peptidase -
J8668_RS13405 (J8668_13405) 2876203..2877012 - 810 WP_003413193.1 shikimate dehydrogenase -
J8668_RS13410 (J8668_13410) 2877009..2878262 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
J8668_RS13415 (J8668_13415) 2878255..2878767 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T198669 WP_003413180.1 NZ_CP072765:c2875119-2874724 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T198669 NZ_CP096945:c4785426-4785127 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT198669 WP_003413183.1 NZ_CP072765:c2875361-2875116 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT198669 NZ_CP096945:c4785130-4784840 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References