Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1948602..1949215 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag J8668_RS09010 Protein ID WP_003408465.1
Coordinates 1948602..1948991 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag J8668_RS09015 Protein ID WP_003408469.1
Coordinates 1948988..1949215 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS08980 (J8668_08980) 1944231..1945145 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
J8668_RS08985 (J8668_08985) 1945148..1945978 + 831 WP_003916848.1 cyclase family protein -
J8668_RS08990 (J8668_08990) 1945978..1946328 + 351 WP_003898986.1 cupin domain-containing protein -
J8668_RS08995 (J8668_08995) 1946381..1947199 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
J8668_RS09000 (J8668_09000) 1947213..1947992 + 780 WP_003408460.1 IclR family transcriptional regulator -
J8668_RS20765 1948423..1948554 + 132 Protein_1799 IS3 family transposase -
J8668_RS09010 (J8668_09010) 1948602..1948991 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
J8668_RS09015 (J8668_09015) 1948988..1949215 - 228 WP_003408469.1 antitoxin Antitoxin
J8668_RS09020 (J8668_09020) 1949433..1950917 + 1485 WP_003408473.1 biotin carboxylase -
J8668_RS09025 (J8668_09025) 1950914..1952161 + 1248 WP_003408476.1 serine hydrolase -
J8668_RS09030 (J8668_09030) 1952204..1952623 - 420 WP_003408483.1 hypothetical protein -
J8668_RS09035 (J8668_09035) 1952613..1953323 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T198652 WP_003408465.1 NZ_CP072765:c1948991-1948602 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T198652 NZ_CP096941:c5865085-5864807 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT198652 WP_003408469.1 NZ_CP072765:c1949215-1948988 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT198652 NZ_CP096941:c5864795-5864490 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References