Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 694842..695488 Replicon chromosome
Accession NZ_CP072765
Organism Mycobacterium tuberculosis strain H37Rv_CG

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag J8668_RS03100 Protein ID WP_003403122.1
Coordinates 694842..695234 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag J8668_RS03105 Protein ID WP_003403125.1
Coordinates 695231..695488 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8668_RS03085 (J8668_03085) 690504..692030 + 1527 WP_003403112.1 virulence factor Mce family protein -
J8668_RS03090 (J8668_03090) 692093..693235 + 1143 WP_003911253.1 virulence factor Mce family protein -
J8668_RS03095 (J8668_03095) 693240..694790 + 1551 WP_003403119.1 MlaD family protein -
J8668_RS03100 (J8668_03100) 694842..695234 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
J8668_RS03105 (J8668_03105) 695231..695488 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
J8668_RS03110 (J8668_03110) 695671..696906 - 1236 WP_003403128.1 ATP-binding protein -
J8668_RS03115 (J8668_03115) 697157..697570 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
J8668_RS03120 (J8668_03120) 697567..697803 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
J8668_RS03125 (J8668_03125) 697907..698413 - 507 WP_003900973.1 two component system sensor kinase HK1 -
J8668_RS03130 (J8668_03130) 698527..699057 - 531 WP_003917320.1 two component system sensor kinase HK2 -
J8668_RS03135 (J8668_03135) 699041..699736 - 696 WP_003918099.1 two-component system response regulator TcrA -
J8668_RS03140 (J8668_03140) 699859..700170 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T198630 WP_003403122.1 NZ_CP072765:c695234-694842 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T198630 NZ_CP096929:c5817530-5817252 [Pseudomonas aeruginosa]
ATGATTCTGGCCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT198630 WP_003403125.1 NZ_CP072765:c695488-695231 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT198630 NZ_CP096929:c5817240-5816935 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References