Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3188134..3188821 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag J8671_RS15080 Protein ID WP_003414624.1
Coordinates 3188378..3188821 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag J8671_RS15075 Protein ID WP_003414620.1
Coordinates 3188134..3188391 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS15055 (J8671_15055) 3183454..3184308 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
J8671_RS15060 (J8671_15060) 3184364..3185527 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
J8671_RS15065 (J8671_15065) 3185544..3186758 - 1215 WP_003899518.1 zinc metalloprotease Rip -
J8671_RS15070 (J8671_15070) 3186766..3188007 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
J8671_RS15075 (J8671_15075) 3188134..3188391 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
J8671_RS15080 (J8671_15080) 3188378..3188821 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
J8671_RS15085 (J8671_15085) 3188901..3189563 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
J8671_RS15090 (J8671_15090) 3189659..3189850 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
J8671_RS15095 (J8671_15095) 3190182..3191930 + 1749 WP_178121166.1 cytochrome c biogenesis protein DipZ -
J8671_RS15100 (J8671_15100) 3192026..3192607 + 582 WP_003414644.1 fasciclin domain-containing protein -
J8671_RS15105 (J8671_15105) 3192707..3192973 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T198617 WP_003414624.1 NZ_CP072764:3188378-3188821 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T198617 NZ_CP096927:c145894-145613 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGAAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT198617 WP_003414620.1 NZ_CP072764:3188134-3188391 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT198617 NZ_CP096927:c146118-145891 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAAAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References