Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-Phd
Location 3182533..3183081 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name relE Uniprot ID G0TFG5
Locus tag J8671_RS15050 Protein ID WP_003414602.1
Coordinates 3182818..3183081 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID O33347
Locus tag J8671_RS15045 Protein ID WP_003414599.1
Coordinates 3182533..3182814 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS15020 (J8671_15020) 3178156..3179013 - 858 WP_003899513.1 type I methionyl aminopeptidase -
J8671_RS15025 (J8671_15025) 3179055..3179639 - 585 WP_003899514.1 DUF1707 domain-containing protein -
J8671_RS15030 (J8671_15030) 3179743..3179991 + 249 WP_003913411.1 antitoxin VapB23 -
J8671_RS15035 (J8671_15035) 3179988..3180368 + 381 WP_003414592.1 type II toxin-antitoxin system VapC family toxin -
J8671_RS15040 (J8671_15040) 3180450..3182261 - 1812 WP_003414596.1 penicillin-binding protein -
J8671_RS15045 (J8671_15045) 3182533..3182814 + 282 WP_003414599.1 type II toxin-antitoxin system antitoxin RelF Antitoxin
J8671_RS15050 (J8671_15050) 3182818..3183081 + 264 WP_003414602.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8671_RS15055 (J8671_15055) 3183454..3184308 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
J8671_RS15060 (J8671_15060) 3184364..3185527 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
J8671_RS15065 (J8671_15065) 3185544..3186758 - 1215 WP_003899518.1 zinc metalloprotease Rip -
J8671_RS15070 (J8671_15070) 3186766..3188007 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10204.82 Da        Isoelectric Point: 11.5078

>T198616 WP_003414602.1 NZ_CP072764:3182818-3183081 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Download         Length: 264 bp

>T198616 NZ_CP096923:c3496364-3496146 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10183.40 Da        Isoelectric Point: 4.3440

>AT198616 WP_003414599.1 NZ_CP072764:3182533-3182814 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Download         Length: 282 bp

>AT198616 NZ_CP096923:c3496767-3496393 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3G5O

Antitoxin

Source ID Structure
PDB 3G5O
AlphaFold DB A0A7U4BWP8

References