Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3141616..3142220 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag J8671_RS14850 Protein ID WP_003414492.1
Coordinates 3141616..3142008 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P71622
Locus tag J8671_RS14855 Protein ID WP_003912003.1
Coordinates 3142005..3142220 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS14820 (J8671_14820) 3136766..3137554 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
J8671_RS14825 (J8671_14825) 3137888..3138433 - 546 WP_003913758.1 DUF1802 family protein -
J8671_RS14830 (J8671_14830) 3138705..3139589 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
J8671_RS14835 (J8671_14835) 3139592..3140479 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
J8671_RS14840 (J8671_14840) 3140784..3141329 - 546 WP_003899500.1 DUF1802 family protein -
J8671_RS14845 (J8671_14845) 3141326..3141595 - 270 WP_003414489.1 DUF2277 family protein -
J8671_RS14850 (J8671_14850) 3141616..3142008 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
J8671_RS14855 (J8671_14855) 3142005..3142220 - 216 WP_003912003.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J8671_RS14860 (J8671_14860) 3142267..3143016 + 750 WP_003414497.1 enoyl-CoA hydratase -
J8671_RS14865 (J8671_14865) 3143095..3144177 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
J8671_RS14870 (J8671_14870) 3144170..3145480 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
J8671_RS14875 (J8671_14875) 3145483..3146310 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
J8671_RS14880 (J8671_14880) 3146307..3147218 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T198615 WP_003414492.1 NZ_CP072764:c3142008-3141616 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T198615 NZ_CP096923:c2340495-2340124 [Enterobacter hormaechei]
ATGGAGCACATGGCTGTGTTTGACACCAATATCCTCATCGATCTTTTTAATAACCACGTTGAGGCTGCGGATGCGATCGA
TCGCACAGCTTCGCATCGTGCCATTAGCCTTATCACATGGATGGAAGTAATGGTGGGCGCTCGCAGGCACGGTCAAGAAG
CAAGAACGGCAGCCGTGATGGGGGCCTTTGAAATTATTGATATAACGCGGGAAATTGCTGAAAGAAGCGTATTACTTCGC
GAACAGCACGGGATGAAGCTTCCTGACGCCATTATTCTGGCTACTGCTCAGAGCAGAAGCTGTCCGTTAATTTCGCGCAA
TACGAAAGACTTTTCGGGTATTGCTGGGGTCGTTTCACCTTACCACCTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7483.50 Da        Isoelectric Point: 4.9732

>AT198615 WP_003912003.1 NZ_CP072764:c3142220-3142005 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Download         Length: 216 bp

>AT198615 NZ_CP096923:c2340740-2340498 [Enterobacter hormaechei]
ATGAGTCTTTTGGCGGACCTTACTATGGGCAGAATACTGATCGATTTGTCTGATGACGTGATTCAACGACTGGATAACCT
GAAGCAGTTGCGCAACCGGCCGCGCGCCGAACTGCTGCGGGAAGCCATAGAACTGTATCTCGACCAGCAGAATTCATCGG
TTATCCGCGAAGCGCTTGGCCTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTACGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB P71622

References