Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3115163..3115733 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag J8671_RS14705 Protein ID WP_003414166.1
Coordinates 3115163..3115519 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag J8671_RS14710 Protein ID WP_003901465.1
Coordinates 3115503..3115733 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS14685 (J8671_14685) 3110615..3112303 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
J8671_RS14690 (J8671_14690) 3112307..3112633 - 327 WP_003414157.1 hypothetical protein -
J8671_RS14695 (J8671_14695) 3112806..3113393 + 588 WP_003914429.1 DUF3558 family protein -
J8671_RS14700 (J8671_14700) 3113412..3115061 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
J8671_RS14705 (J8671_14705) 3115163..3115519 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
J8671_RS14710 (J8671_14710) 3115503..3115733 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
J8671_RS14715 (J8671_14715) 3115776..3116819 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
J8671_RS20885 3116818..3117285 + 468 WP_003414177.1 DUF1778 domain-containing protein -
J8671_RS14725 (J8671_14725) 3117461..3117715 - 255 WP_003917684.1 hypothetical protein -
J8671_RS14730 (J8671_14730) 3117863..3118267 + 405 WP_009938577.1 hypothetical protein -
J8671_RS14735 (J8671_14735) 3118264..3118455 + 192 WP_003414184.1 hypothetical protein -
J8671_RS20890 3118672..3118932 + 261 Protein_2946 transposase -
J8671_RS14745 (J8671_14745) 3120042..3120299 + 258 WP_003899489.1 hypothetical protein -
J8671_RS14750 (J8671_14750) 3120404..3120715 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T198613 WP_003414166.1 NZ_CP072764:c3115519-3115163 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T198613 NZ_CP096923:c1528542-1528132 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCAACTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT198613 WP_003901465.1 NZ_CP072764:c3115733-3115503 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT198613 NZ_CP096923:c1528789-1528523 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References